[ccp4bb]: deglycosylating enzymes

[Tom Brett <tbrett@pathbox.wustl.edu>]

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Hello all:
	I am attempting to deglycosylate a protein to hopefully enhance my
success of getting crystals of it.  I have used the GST-PNGase F enzyme
available from Hampton Research and found that (for this protein) it
works well (as deduced from SDS-PAGE).  The problem is that I do not
have much of the PNGase F left and I was just told by Hampton that they
do not have any at this time and they won't have any until sometime in
January.  I would enjoy not having to wait that long, so I was wondering
if anyone had ever used any other enzyme from another vendor for the
purpose of deglycosylating a protein for crystallization?  I don't think
there are any other vendors that offer the enzyme with a GST-tag (or any
other tag for that matter).

Thanks in advance.

-Tom
-- 
Tom J. Brett, Ph. D.
Postdoctoral Researcher
Department of Pathology and Immunology 
Campus Box 8118
Washington University
St. Louis, MO  63110
phone: (314) 747-4546
e-mail: tbrett@pathbox.wustl.edu