Re: [ccp4bb]: protein oil out

[Bart Hazes <bhazes@ualberta.ca>]

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Dear Hongmin,

I have worked with hemocyanins in the past that were highly soluble like
yours. Some of these crystallised by dialysis against a very low ionic
strength buffer. I have no idea if this is a general property of highly
soluble proteins (is there anything general about proteins anyway) but you may
want to consider it.

Bart Hazes


On Tue, 27 Nov 2001, hongmin LI wrote:

> hello,
>
> Sorry this question is not CCP4 related. I am working on a protein which
> is highly soluble. I can easily concentrate it to 60mg/ml. While the
> protein was not precipitated from most chemicals (PEG, (NH4)SO4, molonic
> acid, ...), instead, it is oil out. Does anybody have similar problem
> before? Any suggestions ? Thanks in advance.
>
> Hongmin
>
> --
> -------------------------------
> Hongmin LI, Ph.D.
> Wadsworth Center
> New York State Department of Health
> Empire State Plaza, P.O. Box 509
> Albany, NY 12201-0509
>
> Tel: 518-486-9154
> Fax: 518-474-7992
>
>
>

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