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Re: [ccp4bb]: unusually high solvent content
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Serine hydroxymethyltransferase Vm = 7.7,, solvent content about
85. Membrane proteins often tend to have high Matthew parameters.
***************************************************
** Ulrich Baumann, Dpt. f. Chemie und Biochemie **
** Universitaet Bern, Freiestrasse 3 **
** CH-3012 Bern, Switzerland **
** phone +41-(0)31-631-4320/4324, fax -4887 **
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On Thu, 20 Dec 2001, Manfred Buehner wrote:
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>
> On Wed, 19 Dec 2001, JoaoMDias wrote:
>
> > According to Matthews, (Mathews, B. W. (1968). J. Mol. Biol. 33,
> > 491-497.), the Matthew's coefficient of protein crystals is usually
> > comprehended between 1.7-3.5 A3/Da.
> >
> > Are there many proteins with a much higher solvent content that the
> > Matthews limits?
> >
> > Do you have experience with protein crystals with high solvent contents?
>
> I recall only one, its Matthew coefficient was 4.54 A3/Da.
> The crystals were very fragile and were anisotropically
> disordered, diffracting out to 5 A in one direction and
> to 3 A in the other two (FEBS Letters 1978, 85, 91-94).
>
>
> > Could you point me some references to published works, or reviews about
> > protein crystal structures with unusual VM (above 70%)?
> >
> > Thanks in advance and Merry Christmas,
> > Joao Miguel
>
> MERRY XMAS
>
> Manfred
> ----------------------------------------------------------
> Dr. Manfred Buehner Phone: +49-931-888-4100
> Physiologische Chemie FAX: +49-931-888-4150
> Theodor-Boveri-Institut fuer Biowissenschaften
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>