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Re: [ccp4bb]: unusually high solvent content
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On Wed, 19 Dec 2001, JoaoMDias wrote:
> According to Matthews, (Mathews, B. W. (1968). J. Mol. Biol. 33,
> 491-497.), the Matthew's coefficient of protein crystals is usually
> comprehended between 1.7-3.5 A3/Da.
>
> Are there many proteins with a much higher solvent content that the
> Matthews limits?
>
> Do you have experience with protein crystals with high solvent contents?
I recall only one, its Matthew coefficient was 4.54 A3/Da.
The crystals were very fragile and were anisotropically
disordered, diffracting out to 5 A in one direction and
to 3 A in the other two (FEBS Letters 1978, 85, 91-94).
> Could you point me some references to published works, or reviews about
> protein crystal structures with unusual VM (above 70%)?
>
> Thanks in advance and Merry Christmas,
> Joao Miguel
MERRY XMAS
Manfred
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Dr. Manfred Buehner Phone: +49-931-888-4100
Physiologische Chemie FAX: +49-931-888-4150
Theodor-Boveri-Institut fuer Biowissenschaften
Biozentrum der Universitaet Wuerzburg
Am Hubland, D-97074 WUERZBURG, Germany
Email: buehner@biozentrum.uni-wuerzburg.de
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