Re: [ccp4bb]: unusually high solvent content

[Susan Chacko <susanc@chisos.cit.nih.gov>]

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Tropomyosin crystals have 95% solvent in at least one crystal form. 

See:
Whitby & Phillips, Proteins 2000 Jan 1;38(1):49-59

& the original paper
Phillips et al, Nature 1979 Mar 29;278(5703):413-7


On Wed, 19 Dec 2001, JoaoMDias wrote:

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> 
> Dear All,
> 
> According to Matthews, (Mathews, B. W. (1968). J. Mol. Biol. 33,
> 491-497.), the Matthew's coefficient of protein crystals is usually
> comprehended between 1.7-3.5 A3/Da.
> 
> Are there many proteins with a much higher solvent content that the
> Matthews limits?
> 
> Do you have experience with protein crystals with high solvent contents?
> 
> Could you point me some references to published works, or reviews about
> protein crystal structures with unusual VM (above 70%)?
> 
> Thanks in advance and Merry Christmas,
> Joao Miguel
> 
> 
>