Re: [ccp4bb]: DL-SeMet proper?

["Artem Evdokimov" <AEVDOKIMOZ@cinci.rr.com>]

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DL works just fine, you need an excess over the pure enantiomer, but it does
not hurt. Keep SeMet concentrations in the media under 100 mg/L because
upwards of that it can become toxic.

Artem
----- Original Message -----
From: "李 林森" <y_y_d@hotmail.com>
To: <ccp4bb@dl.ac.uk>
Sent: Thursday, February 28, 2002 8:34 AM
Subject: [ccp4bb]: DL-SeMet proper?


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***          CCP4 home page http://www.ccp4.ac.uk         ***


Hi, all,

It's not something on the topic of CCP4.

I am planning to express a SeMet incorporated protein in E.coli B834(DE3).
One month ago I ordered DL-SeMet from sigma and now it arrived. I had
thought of useing it to do the expression, but I was worrying at the
moment. Is DL-SeMet proper for SeMet incorporated protein expression? Will
the D-SeMet affect the effect and quality of the expression?

As the long-time waiting(about 1 month) for L-SeMet and the surprising
expense of these chemicals, I had not given up using DL-SeMet. Anyone did
expression using DL-SeMet? What about the results? Any suggestions are
wellcom.

Thanks in advance.

sincerely,

Yadong


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