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[ccp4bb]: DL-SeMet proper?

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Hi, all,

It's not something on the topic of CCP4.

I am planning to express a SeMet incorporated protein in E.coli B834(DE3). 
One month ago I ordered DL-SeMet from sigma and now it arrived. I had 
thought of useing it to do the expression, but I was worrying at the 
moment. Is DL-SeMet proper for SeMet incorporated protein expression? Will 
the D-SeMet affect the effect and quality of the expression?

As the long-time waiting(about 1 month) for L-SeMet and the surprising 
expense of these chemicals, I had not given up using DL-SeMet. Anyone did 
expression using DL-SeMet? What about the results? Any suggestions are 
wellcom.

Thanks in advance.

sincerely,

Yadong


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