Re: [ccp4bb]: making movie by Bobscript

[Juergen Bosch <jbosch@biochem.mpg.de>]

Title: Re: [ccp4bb]: making movie by Bobscript

Hi Mary,

how about using dino, writing out a POV Ray file and rendering the scene/movie in Pov Ray ?

Here you can find several binaries:

http://www.biozentrum.unibas.ch/~xray/dino/

http://www.povray.org/

If you want to render surfaces you'll additionally  need the program msms - a link can be found on the dino page for download.

1. Convert your pdb file mit pdb_to_xyzr yourfile.pdb > yourfile.xyzr to a format readable for msms

2. msms -if yourfile.xyzr -of yourfile

This will write out a .vert and a .face file

3. start dino and read in your data e.g:

l

load Yourfile.pdb
...Yourfile new -name all
...Yourfile new -name ca -type trace -selection $protein
...Yourfile.ca set color=yellow
load Yourfile -type msms -name surf
...surf new -name all
scene autoslab

.surf attach ...Yourfile

.surf.all set color=blue -sel $basic
...surf.all set color=blue4 -sel $basic2
...surf.all set color=red -sel $acidic
...surf.all set color=red4 -sel $acidic2
...surf.all set color=cyan -sel $polar
...surf.all set color=green -sel $aromatic
...surf.all set color=yellow -sel $aliphatic
write Yourfile.pov

stop

You might have to center your image correctly in the window by simply clicking on a residue and pressing the right mouse button for the context menu, then select center CP.

4. start povray

5. edit the yourfile.pov file and add the following lines:

 rotate clock*360*x
or

 rotate clock*360*y

light_source {<0,0,200> color rgb <1,1,1>}
light_source {<0,0,-200> color rgb <1,1,1>}
light_source {<0,-200,0> color rgb <1,1,1>}
light_source {<0,200,0> color rgb <1,1,1>}

to have the molecule bathed in light, you might have to change the value 200, dependend on the size of your protein.

And dependent on the size of your movie go and have a coffee or two.

A 320 x 240 Scene with 120 images takes on a PB G3/500 approximately 1 h, this is using virtual memory :-(

I hope I could show you a fast and easy way to produce a nice rendered movie.

(no I'm not paid by anyone of the authors *g*)

Juergen

Juergen Bosch                                                   Tel -49 -89 8578 2648
Max Planck Institute for Biochemistry               Fax -49 -89 8578 2641
Department of Molecular Structural Biology       bosch@biochem.mpg.de
Am Klopferspitz 18a                                         jbosch@biochem.mpg.de
D-82152 Martinsried, Germany