Re: [ccp4bb]: Data reduction for mosaic crystals....

[Ricardo Aparicio <aparicio@if.sc.usp.br>]

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Dear Rajeswari:

Many times MOSFLM (ccp4 program) option "estimate mosaicity" can help 
you to not overestimate your mosaicity. You can analyse it later in 
SCALA (ccp4 program) and come back to MOSFLM in the case you want to 
test other values for mosaicity.

We've processed data with slices of 0.2 deg with MOSFLM without problems.

A reference on super-fine phi-sliced diffraction:

J. Appl. Cryst. (2000). 33, 1187-1188
J. Lovelace, E. H. Snell, M. Pokross, A. S. Arvai, C. Nielsen, N.-H. 
Xuong, H. D. Bellamy and G. E. O. Borgstahl

Hope it helps you.

Ricardo Aparicio
PhD Student
Brazil


raji wrote:

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> 
> Hi,
> I am working on a dataset where the crystal has a very high mosaicity - my 
> estimate is ~2.0 or more. Hence, I have had to take 0.1deg frames during data 
> collection. Denzo V. 1.97.2 does not seem to be scaling the data very well. 
> Would anyone know if HKL2000 can do a good job or if there's any other package 
> thats good with dealing with minislices.
> Also, any suggestions about how to treat and process minislices?
> Thank you very much.
> Raji
> 
> Rajeswari Edayathumangalam
> Dept. of Biochemistry & Molecular Biology
> Colorado State University
> Fort Collins, CO 80523 USA
> 
>