[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
[ccp4bb]: glycosylation
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Here a rather not so crystallographic question for the weekend:
If I'm running a 40 kDa protein with an added glycosylation of about 20
kDa on a gel filtration/permeation column (Superose 6 or Superdex
etc...) do I expect the protein to behave more like a 60 kDa protein or
more like a 40 kDa protein.
I wonder if anybody has experience with the migration of glycosylated
proteins in gel filtration.
Any hints are much appreciated.
Many thanks
Yves
--
------------------------------------------------------------------------
Yves Muller
Department of Biochemistry
School of Biological Sciences Phone: +44-(0)-1273-678515
University of Sussex Fax: +44-(0)-1273-678433
Falmer, Brighton BN1-9QG E-mail: y.muller@sussex.ac.uk
UK http://www.biols.susx.ac.uk/faculty/biochem/muller.htm
------------------------------------------------------------------------