Re: [ccp4bb]: selenium incorporation in Pichia Pastoris

[Frank Von Delft <loretta@scripps.edu>]

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Try this one:

	Larsson AM, Stahlberg J, Jones TA (2002)  "Preparation and
crystallization of selenomethionyl dextranase from Penicillium minioluteum
expressed in Pichia pastoris"  Acta Cryst D58, 346-348.

phx.



On Tue, 7 Jan 2003, Goetz wrote:

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> Dear Structural Biologists,
> 	Apologies for the non-ccp4 related question. The conventional
> wisdom here seems to be that selenomethionine incorporation in Pichia does
> not work. Can that possibly be true? I've done some poking around and
> found a few positive stories but details are lacking. References and or
> anecdotal evidence of both success and failure would be appreciated.
>
> Thanks,
> Dave Goetz
>
>
>