Re: [ccp4bb]: What has been will be again,what has been done will be done again; there is nothing new under the sun.

[Jan Dohnalek <jd23@york.ac.uk>]

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Wonderful. So who goes ahead and patents the hanging drop method? :-)
And may be also how to inspect crystals under the microscope ....
Jan



"Dunten, Pete {IVDU~Palo Alto}" wrote:
> 
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> 
> An interesting patent application has recently been published (US20030049678 A1).  The beginning of the claims section is below, another fine example of Abbott innovation.
> 
> We claim:
> 1. A process for identifying a ligand to a target biomolecule comprising, a) obtaining a target biomolecule crystal; b) exposing the target biomolecule crystal to one or more test samples; and c) obtaining an X-ray crystal diffraction pattern to determine whether a ligand/receptor complex is formed.
> 2. The process according to claim 1 further comprising the steps of obtaining an X-ray crystal diffraction pattern of the target biomolecule crystal prior to exposure to the test samples and comparing the X-ray diffraction pattern of the target molecule before and after the exposure.
> 3. The process according to claim 1 further comprising the step of transforming diffraction pattern into an electron density map.
> 4. The process according to claim 3 further comprising the step of converting electron density map into a structure.
> 5. The process according to claim 1, wherein the target biomolecule is exposed to a test sample by soaking the target biomolecule crystal in a solution that contains the test sample.

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Jan Dohnalek
York Structural Biology Laboratory
Department of Chemistry
York
YO105YW

E-mail: jd23@york.ac.uk
Phone: +44 1904 328270  Fax: +44 1904 328266

(Institute of Macromolecular Chemistry, Academy of Sciences
of the Czech Republic, Prague, Czech Republic)
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