Re: [ccp4bb]: Protein_DNA complex

[William Scott <wgscott@chemistry.ucsc.edu>]

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On Thursday, March 27, 2003, at 08:00 PM, matthew hogg wrote:

>>> Is there a clever way to tell if our crystals are complexed 
>>> protein:DNA
>>> versus unbound protein, without having to wait until we have maps? 
>>> We have a
>>> fluorophore attached to the DNA fragment.


Sorry, I should have read this more carefully.  If your protein 
contains tryptophan or possibly other aromatics you might be able to do 
a single-crystal FRET experiment if your fluorophore emits light at a 
wavelength that an aromatic residue might be able to absorb (or vice 
versa).