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Re: [ccp4bb]: Protein_DNA complex
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On Thursday, March 27, 2003, at 08:00 PM, matthew hogg wrote:
>>> Is there a clever way to tell if our crystals are complexed
>>> versus unbound protein, without having to wait until we have maps?
>>> We have a
>>> fluorophore attached to the DNA fragment.
Sorry, I should have read this more carefully. If your protein
contains tryptophan or possibly other aromatics you might be able to do
a single-crystal FRET experiment if your fluorophore emits light at a
wavelength that an aromatic residue might be able to absorb (or vice