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Re: [ccp4bb]: Enzymatic reaction inside protein crystals
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Ola Francisco,
A problem very similar to yours which was demonstrated in a very elegant
way, was recently published in Nature by Hajdu and co-workers. See
reference:
Berglund et al. (2002): The catalytic pathway of horseradish peroxidase at
high resolution. Nature, 417, 463-468.
Which kind of reaction do you see? Reduction by X-rays?
Best regards,
Joao Miguel
******************************************************************
Joao M. Dias, Ph.D.
Protein Crystallography
Dep. Quimica, CQFB email: jmdias@dq.fct.unl.pt
Fac. Ciencias Tecnologia Tel: +351-212948300 ext:10962
Universidade Nova Lisboa Fax: +351-212948550
2829-516 Caparica
Portugal
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"Francisco J. Enguita" wrote:
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> *** CCP4 home page http://www.ccp4.ac.uk ***
>
> Dear all :
>
> First of all, sorry for posting this non-CCP4 question, but perhaps
> someone could help me.
>
> I am dealing with an enzyme (oxidase) which is able to react with its
> substrate inside protein crystals. I have some data about the reaction
> mechanism of the enzyme, but, is anybody dealing with a situation like
> this ?. I would like to share data about other enzymes which are "still
> active" inside crystals.
>
> Thank you.
>
> Cheers
>
> Francisco
>
> --
>
> ============================================
> Francisco J. Enguita, Ph.D.
> Protein Crystallography laboratory
> Instituto de Tecnologia Qummica e Biolsgica
> 2781-901 Oeiras
> PORTUGAL
> Phone : 351-21-4469669
> Fax : 351-21-4433644
> E-mail : fenguita@itqb.unl.pt
> ============================================