Re: [ccp4bb]: Protein-DNA complex

[Frank Murphy <fm@mrc-lmb.cam.ac.uk>]

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I would suggest rinsing a few crystals and running on an SDS-PAGE. UV 
shadow for the DNA then silver stain for the protein. I have actually 
observed slight negative staining where DNA runs. In my experience this is 
the quickest way to get the answer Acta Cryst. (1999). D55, 1594-1597 
(figure 1)


>X-DL-MFrom: <wgscott@chemistry.ucsc.edu>
>X-DL-Connect: <hydrogen.ucsc.edu [128.114.41.29]>
>Date: Thu, 27 Mar 2003 19:47:45 -0800
>Subject: Re: [ccp4bb]: Protein-DNA complex
>From: William Scott <wgscott@chemistry.ucsc.edu>
>To: ccp4bb@dl.ac.uk
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>A very good dye for nucleic acids is toluidine blue.  You just make a 0.5 
>% solution in water, stain your gel (say 20% acrylamide) for 10 minutes, 
>and then rinse it off with hot water.  Remember though that anything that 
>stains DNA is likely a carcinogen.  Also, you can phosphorylate the DNA 
>with 32P either before or after crystallization and that will be rather 
>unambiguous.
>
>
>

Dr. Frank V. Murphy Ph.D.
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